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AssigneeHenkel AG & Co. KGaA
InventorsNina Mussmann, Timothy O'Connell, Daniela Herbst, Inken Prueser, Christian Degering, Sabine Griemert, Thorsten Eggert, Christian Leggewie
Estimating the term…
The present disclosure relates to proteases having an amino acid sequence with at least about 70% sequence identity to the amino acid sequence given in SEQ ID NO:2, over the entire length thereof, and comprising an amino acid substitution at at least one of the positions P9, Q62, D101, N130, G166, N187, 5216, N238 or Q271, based in each case on the numbering according to SEQ ID NO:2. The present disclosure also relates to the production and use thereof. Such proteases exhibit very good stability, in particular storage stability, while at the same time having a good cleaning performance.
A protease having an amino-acid sequence which has an at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2 over the entire length thereof and which has an amino acid substitution at one or more of the positions P9, Q62, D101, N130, G166, N187, S216, N238 and Q271, each based on the numbering according to SEQ ID NO:2.
A method for preparing a protease, comprising substituting an amino acid at one or more of the positions which correspond to positions 9, 62, 101, 130, 166, 187, 216, 238 and 271 in SEQ ID NO:2 in a starting protease of which the sequence is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:2 over the entire length thereof.
Dependencies are inferred from the claim text.