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US 10,766,960 B2Granted
Heterodimerized polypeptide
AssigneeChugai Seiyaku Kabushiki Kaisha
InventorsTomoyuki Igawa, Futa Mimoto, Hitoshi Katada, Hirotake Shiraiwa
Life of this patent
Estimating the term…
Abstract
The present inventors produced a heterodimerized polypeptide having an Fc region formed from two polypeptides with different amino acid sequences (a first polypeptide and a second polypeptide), and succeeded in producing a heterodimerized polypeptide containing an Fc region with improved function compared to that of a homodimer in which the Fc region is composed of only the first polypeptide or the second polypeptide by conventional technology.
Claims17
A heterodimer comprising a first polypeptide and a second polypeptide,
- wherein the first polypeptide comprises an IgG Fc region comprising a set of amino acids selected from the group consisting of sets (a) to (aj) below (all positions by EU numbering):
- (a) amino acid E at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- amino acid D at position 327;
- (b) amino acid S at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (c) amino acid L at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (d) amino acid L at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (e) amino acid S at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (f) amino acid F at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (g) amino acid E at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (h) amino acid F at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (i) amino acid V at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (j) amino acid D at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (k) amino acid Q at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (l) amino acid I at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (m) amino acid M at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (n) amino acid T at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (o) amino acid A at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (p) amino acid G at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (q) amino acid H at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (r) amino acid V at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (s) amino acid D at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (t) amino acid Q at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (u) amino acid I at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (v) amino acid M at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (w) amino acid T at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (x) amino acid A at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (y) amino acid G at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (z) amino acid H at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid M at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (aa) amino acid F at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (ab) amino acid E at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (ac) amino acid D at position 234;
- amino acid Q at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid E at position 270;
- and amino acid A at position 298;
- (ad) amino acid V at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (ae) amino acid I at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (af) amino acid L at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (ag) amino acid E at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (ah) amino acid D at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- (ai) amino acid F at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- and
- (aj) amino acid T at position 234;
- amino acid Y at position 235;
- amino acid W at position 236;
- amino acid I at position 239;
- amino acid D at position 268;
- amino acid A at position 298;
- and amino acid D at position 327;
- and
- the second polypeptide comprises an IgG Fc region that is different from the Fc region of the first polypeptide and comprises a set of amino acids selected from the group consisting of sets (ak) to (aq) (all positions by EU numbering):
- (ak) amino acid E at position 270;
- amino acid D at position 326;
- amino acid K at position 330;
- and amino acid E at position 334;
- (al) amino acid E at position 270;
- amino acid D at position 326;
- amino acid M at position 330;
- and amino acid E at position 334;
- (am) amino acid E at position 270;
- amino acid D at position 326;
- amino acid A at position 330;
- and amino acid E at position 334;
- (an) amino acid E at position 270;
- amino acid D at position 326;
- amino acid F at position 330;
- and amino acid E at position 334;
- (ao) amino acid E at position 270;
- amino acid D at position 326;
- amino acid I at position 330;
- and amino acid E at position 334;
- (ap) amino acid E at position 270;
- amino acid D at position 326;
- amino acid Y at position 330;
- and amino acid E at position 334;
- and
- (aq) amino acid E at position 270;
- amino acid D at position 326;
- amino acid H at position 330;
- and amino acid E at position 334.
282 more elements
Dependencies are inferred from the claim text.