Methods, compositions and kits for treating a subject using a recombinant neutralizing binding protein
AssigneeTufts University
InventorCharles B. Shoemaker
Life of this patent
Estimating the term…
Abstract
Methods, compositions and kits are provided for treating a subject exposed to or at risk for exposure to a disease agent using a pharmaceutical composition including at least one recombinant binding protein or a source of expression of the binding protein, wherein the binding protein neutralizes a disease agent that is a toxin, for example a Clostridium difficile toxin, a Shiga toxin, a ricin toxin, or an anthrax toxin.
Claims13
An isolated recombinant binding protein comprising an amino acid sequence selected from SEQ ID NO:
- 100, SEQ ID NO:
- 102, SEQ ID NO:
- 104, SEQ ID NO:
- 106, SEQ ID NO:
- 108, SEQ ID NO:
- 110, SEQ ID NO:
- 112, SEQ ID NO:
- 114, SEQ ID NO:
- 116, SEQ ID NO:
- 118, SEQ ID NO:
- 120, or SEQ ID NO:
- 122, which specifically binds to Anthrax protective antigen (PA) toxin.
A composition comprising at least one recombinant binding protein that specifically binds to Anthrax protective antigen (PA) toxin, wherein the at least one binding protein comprises an amino acid sequence selected from SEQ ID NO:
- 100, SEQ ID NO:
- 102, SEQ ID NO:
- 104, SEQ ID NO:
- 106, SEQ ID NO:
- 108, SEQ ID NO:
- 110, SEQ ID NO:
- 112, SEQ ID NO:
- 114, SEQ ID NO:
- 116, SEQ ID NO:
- 118, SEQ ID NO:
- 120, or SEQ ID NO:
- 122.
A composition comprising at least one recombinant binding protein that specifically binds to Anthrax protective antigen (PA) toxin, wherein the at least one binding protein is encoded by a polynucleotide sequence comprising a nucleotide sequence selected from SEQ ID NO:
- 101, SEQ ID NO:
- 103, SEQ ID NO:
- 105, SEQ ID NO:
- 107, SEQ ID NO:
- 109, SEQ ID NO:
- 111, SEQ ID NO:
- 113, SEQ ID NO:
- 115, SEQ ID NO:
- 117, SEQ ID NO:
- 119, SEQ ID NO:
- 121, or SEQ ID NO:
- 123.
A multimeric binding protein comprising at least two recombinant camelid heavy-chain-only antibody monomers (VHHs) which specifically bind Anthrax protective antigen (PA) toxin and which comprise an amino acid sequence selected from SEQ ID NO:
- 100, SEQ ID NO:
- 102, SEQ ID NO:
- 104, SEQ ID NO:
- 106, SEQ ID NO:
- 108, SEQ ID NO:
- 110, SEQ ID NO:
- 112, SEQ ID NO:
- 114, SEQ ID NO:
- 116, SEQ ID NO:
- 118, SEQ ID NO:
- 120, or SEQ ID NO:
- 122;
- wherein the at least two VHHs are separated by spacers.
Dependencies are inferred from the claim text.