Multiple antigen presenting immunogenic composition, and methods and uses thereof
AssigneeChildren's Medical Center Corporation
InventorsRichard Malley, Yingjie Lu, Fan Zhang
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Abstract
The present embodiments provide for an immunogenic multiple antigen presenting system comprising a polymer to which antigens are associated by complementary affinity molecules. For example, the polymer can be a polysaccharide, or antigenic polysaccharide, to which protein or peptide antigens from the same or different pathogens are indirectly linked. The present immunogenic compositions can elicit both humoral and cellular immune responses to one or multiple antigens at the same time.
Claims42
An immunogenic composition comprising an immunologically effective amount of at least one antigenic polysaccharide, an immunologically effective amount of one to ten different peptide or polypeptide antigens, and one to ten different pairs of affinity molecules, wherein each pair comprises a first affinity molecule and a second affinity molecule complementary to the first affinity molecule, wherein in each pair:
- the first affinity molecule is associated with the at least one antigenic polysaccharide, and
- the complementary second affinity molecule is covalently attached to one of the peptide or polypeptide antigens to form a fusion protein, and
- the first affinity molecule non-covalently associates with the complementary second affinity molecule to link the respective peptide or polypeptide antigen and the at least one antigenic polysaccharide;
- and
- wherein the immunogenic composition, upon administration to a subject, elicits (i) an immune response to the at least one antigenic polysaccharide, and (ii) an immune response to at least one of the one to ten different peptide or polypeptide antigens, in the subject.
An immunogenic composition comprising:
- an immunologically effective amount of at least one antigenic polysaccharide, and
- an immunologically effective amount of one to ten different peptide or polypeptide antigens,
- wherein the at least one antigenic polysaccharide is cross-linked or covalently bonded to biotin, and the one to ten peptide or polypeptide antigens are associated with at least one rhizavidin peptide, derivative, or functional portion thereof, and
- wherein the one to ten peptide or polypeptide antigens and the at least one antigenic polysaccharide are linked by a non-covalent, affinity interaction between the biotin and the rhizavidin peptide, derivative, or functional portion thereof;
- and
- wherein the immunogenic composition, upon administration to a subject, elicits (i) an immune response to the at least one antigenic polysaccharide, and (ii) an immune response to at least one of the one to ten different peptide or polypeptide antigens, in the subject.
Dependencies are inferred from the claim text.