Methods and compositions to increase the rate of ligation reactions catalyzed by a sortase
AssigneeThe Regents of the University of California
InventorsRobert T. Clubb, Brendan R. Amer
Life of this patent
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Abstract
In various embodiments methods and compositions to increase the rate and/or activity of ligation reactions catalyzed by a sortase. In certain embodiments reagents are provided that comprises, inter alia, a polypeptide comprising an amino terminal polyglycine sequence comprising at least three contiguous Gly residues (e.g., a triglycine sequence, a tetraglycine sequence, a pentaglycine sequence, etc.) followed by a peptide that is to be ligated to a moiety followed by a sequence comprising the catalytic domain of a Sortase A enzyme.
Claims17
A reagent for ligating a desired polypeptide to a target moiety having a linking peptide attached thereto where the linking peptide comprises an LPXTG (SEQ ID NO:
- 2) amino acid motif in the carboxyl terminal of the linking peptide, said reagent comprising:
- a polypeptide comprising an amino terminal polyglycine sequence comprising at least three contiguous Gly residues sequence followed by said desired polypeptide sequence followed by a sequence comprising the catalytic domain of a Sortase A enzyme.
Dependencies are inferred from the claim text.