Method for improving quality of aquaculture pond water using a nutrient germinant composition and spore incubation method

A method of adding bacteria to water used in an aquaculture application, the method comprising:

• providing a volume of nutrient germinant composition and a volume of bacteria spores, which may be premixed together as a nutrient spore composition or separate;
• optionally mixing a portion of the nutrient germinant composition and a portion of the bacteria spores if separate to form the nutrient spore composition;
• heating a portion of the nutrient spore composition to a temperature in a range of around 38° C. to 60° C. at or near a site of the aquaculture application;
• maintaining the temperature in the range for an incubation period of around 2 minutes to around 6 hours to form a batch of germinated bacteria solution;
• periodically repeating the heating and maintaining steps to form additional batches of germinated bacteria solution over the course of a treatment cycle;
• dispersing each batch of germinated bacteria solution into the water used in the aquaculture application;
• providing a nitrification enhancement agent;
• dispersing the nitrification enhancement agent in the water contemporaneously with at least one of the batches of germinated bacteria solution;
• and
• wherein the bacteria are useful for remediating the water by degrading organic waste and inhibiting the growth of pathogenic bacteria or the bacteria are probiotic for a species in the aquaculture application.