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US 10,766,034 B2Granted
Optical cavity PCR
AssigneeThe Regents of the University of California
InventorsLuke P. Lee, Jun Ho Son
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Abstract
An LED-driven optical cavity PCR system and method is disclosed for fast, accurate and reliable PCR based diagnostics. An optical cavity comprising two thin light absorbing metal (AU) films is used for uniform light absorption and subsequent photo thermal light-to-heat conversion is employed for PCR thermal cycling.
Claims9
A method for performing ultra fast thermal cycling of a fluidic sample, the method comprising:
- providing a micro-fluidic thermal cycling chamber defined by opposing first and second thin films;
- filling the micro-fluidic thermal cycling chamber with the fluidic sample;
- illuminating the first thin film with a light source;
- wherein a first portion of light illuminated onto the first thin film is absorbed into the first thin film and a second portion of the light illuminated onto the first thin film is transmitted through the first thin film;
- illuminating the second thin film with the light transmitted through the first thin film;
- wherein at least a portion of the transmitted light illuminating the second thin film is absorbed into the second thin film;
- uniformly elevating a temperature of the first thin film and a temperature of the second thin film as a function of the absorbed light into the first thin film and second thin film;
- and
- heating the fluidic sample within the micro-fluidic thermal cycling chamber as a result of the elevated temperature of the first thin film and second thin film.
6 more elements
Dependencies are inferred from the claim text.