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US 10,765,695 B2Granted
Methods and compositions for selecting siRNA of improved functionality
AssigneeThermo Fisher Scientific Inc.
InventorsAnastasia Khvorova, Angela Reynolds, Devin Leake, William Marshall, Steven Read, Stephen Scaringe
Life of this patent
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Abstract
Efficient sequence specific gene silencing is possible through the use of siRNA technology. Be selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods compositions, and kits generated through rational design of siRNAs are disclosed, including those directed to the nucleotide sequences for AAT.
Claims12
An siRNA molecule, wherein said siRNA molecule consists of:
- (a) a duplex region;
- and (b) either no overhang regions or at least one overhang region, wherein each overhang region contains six or fewer nucleotides, wherein the duplex region consists of a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said sense region and said antisense region are each 19-30 nucleotides in length and said antisense region comprises a sequence that is the complement of SEQ ID NO:
- 517.
A chemically synthesized double stranded siRNA molecule, wherein:
- (a) each strand of said double stranded siRNA molecule is 19-30 nucleotides in length;
- and
- (b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO:
- 517.
1 more element
Dependencies are inferred from the claim text.