Method for improving the mean dry shoot weight, mean dry grain weight, and suppressing seed-borne infection in a cereal crop
AssigneesThe Provost, Fellows, Foundation Scholars, and the Other Members of Board, of the College of the Holy and Undivided Trinity of Queen Elizabeth Near Dublin, University College Dublin, National University of Ireland, Dublin
InventorsBrian Murphy, Trevor Hodkinson, Fiona Doohan
Life of this patent
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Abstract
A seed coating composition comprising a mixture of a fungal root endophyte isolated from a root of the plant found in low-nutrient, drought-stressed or multiply-stressed conditions and a carrier medium for application of said composition to a target seed, wherein the endophyte is characterised in having a nuclear ribosomal internal transcribed spacer (nr ITS) with at least a 90% sequence identity to any one of SEQ ID NOs: 3 to 15.
Claims20
A seed coating composition for use in (a) increasing grain yield and dry shoot weight in a plant, the composition comprising a mixture of a fungal root endophyte isolated from a root of the plant and a carrier medium for application of said composition to a target seed, or for (b) increasing tolerance to the development of seed-borne fungal infections on germinated and ungerminated seeds, the composition comprising a mixture of a fungal root endophyte isolated from a root of a plant sprouted from said seeds and a carrier medium for application of said composition to a target seed, wherein the endophyte is characterised in having a nuclear ribosomal internal transcribed spacer (nrITS) with 100% sequence identity to any one of SEQ ID NOs:
- 3 to 15, wherein the fungal root endophyte is isolated from a root of the plant Hordeum murinum, and wherein the plant and seeds are in low-nutrient conditions, or in drought-stressed conditions, or in multiply-stressed conditions.
A method of preparing a seed having (a) increased grain yield and dry shoot weight or (b) with increased tolerance to the development of seed-borne fungal infections on germinated and ungerminated seeds, the method comprising the step of inoculating the seed with a fungal root endophyte isolated from a root of a plant which produced said seed, wherein the endophyte is characterised in having a nuclear ribosomal internal transcribed spacer (nrITS) with 100% sequence identity to any one of SEQ ID NOs:
- 3 to 15, wherein the fungal root endophyte is isolated from a root of the plant Hordeum murinum, and wherein the plant and seeds are in low-nutrient conditions, or in drought-stressed conditions, or in multiply-stressed conditions.
Dependencies are inferred from the claim text.