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US 10,731,132 B2Granted
Methods and apparatuses for umbilical cord blood collection and isolation of cells
AssigneeAmniotics AB
InventorsMarcus Kare Torleif Larsson, Andreas Nils Walter Herbst, Niels-bjarne Roland Woods
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Abstract
The invention relates to method for the separation of cord blood endothelial cells from hematopoietic cells in a manner that preserves the hematopoietic cells' repopulating ability and primitivity, without significantly reducing the yield of hematopoietic cells. The said endothelial cells from cord blood have a utility in cell based therapeutics. The system described takes advantage of the endogenous adherence properties of the cells to facilitate the separation and separate storage of the cell types.
Claims20
A method for cell separation, comprising:
- plating a freshly harvested umbilical cord blood sample that has not been enriched, stored, thawed, or washed on a surface at a surface density of approximately 100 million cells per 100 cm2, the surface coated in a component configured to selectively bind with endothelial cells within the whole umbilical cord blood sample;
- allowing adhesion of cells in said umbilical cord blood sample to said surface for about 5 minutes to about 60 minutes, to generate an adherent fraction and a non-adherent fraction of said whole umbilical cord blood sample;
- separating said non-adherent fraction from said adherent fraction, wherein said non-adherent fraction comprises a hematogenic progenitor cell or a hematopoietic stem cell;
- wherein said adherent fraction comprises at least one of an endothelial cell and an endothelial progenitor cell;
- and
- processing and storing the non-adherent fraction.
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Dependencies are inferred from the claim text.