Amplification and detection of nucleic acids in a biological sample
AssigneeQuest Diagnostics Investments Incorporated
InventorsLucien Jacky, Albert Castro, Peter Lee, Jose Matud, Michelle Tabb
Life of this patent
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Abstract
Disclosed are methods for identifying the presence or absence of a target nucleic acid from one or more organisms in a biological sample, said method comprising: (a) spinning the sample at a rotational velocity sufficient to pellet cellular debris and fluorescence inhibitors present within the sample and reduce fluorescence interference or quenching in the sample; and (b) directly amplifying and detecting the target nucleic acid in the sample.
Claims18
A method for identifying the presence or absence of a target nucleic acid in a biological sample, the method comprising:
- (a) directly amplifying and detecting a target nucleic acid in a biological sample containing whole blood using a real-time polymerase chain reaction (PCR) assay, wherein the target nucleic acid is not extracted from the biological sample prior to amplifying;
- and
- (b) rotating a biological sample during one or more steps of the real-time PCR assay at a rotational velocity of greater than 140×g and less than or about 1500×g, wherein the rotational velocity is sufficient to pellet cellular debris and fluorescence inhibitors present within the sample to reduce fluorescence interference or quenching in the sample.
The method of 1 claim, wherein the biological sample is placed in a microfluidic disc capable of being subjected to centrifugal force.
Dependencies are inferred from the claim text.