Methods for producing 3-hydroxy-3-methylbutyric acid
AssigneeGlobal Bioenergies
InventorsPhilippe Marlière, Maria Anissimova, Mathieu Allard
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Abstract
Described is a method for the conversion of 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyric acid comprising the steps of: (a) enzymatically converting 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyryl-CoA; and (b) further enzymatically converting the thus produced 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid wherein the enzymatic conversion of 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyric acid according to step (b) is achieved by first converting 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyryl phosphate and then subsequently converting the thus produced 3-hydroxy-3-methylbutyryl phosphate into 3-hydroxy-3-methylbutyric acid.
Claims16
A method for the conversion of 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyric acid comprising the steps of:
- (a) enzymatically converting 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyryl-CoA by a first enzyme having at least 95% sequence identity to one of SEQ ID NOs:1-3 or 6 wherein said first enzyme is capable of converting 3-methylcrotonyl-CoA into 3-hydroxy-3-methylbutyryl-CoA;
- and
- (b) enzymatically converting the thus produced 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyryl phosphate by a second enzyme having at least 95% sequence identity to one of SEQ ID NOs:28 or 29 wherein said second enzyme is capable of converting 3-hydroxy-3-methylbutyryl-CoA into 3-hydroxy-3-methylbutyryl phosphate;
- and
- (c) enzymatically converting the thus produced 3-hydroxy-3-methylbutyryl phosphate into 3-hydroxy-3-methylbutyric acid by a third enzyme having at least 95% sequence identity to one of SEQ ID NOS:30 or 31 wherein said third enzyme is capable of converting 3-hydroxy-3-methylbutyryl phosphate into 3-hydroxy-3-methylbutyric acid.
Dependencies are inferred from the claim text.