Fluid transfer device and process of aseptically transferring a fluid

A fluid transfer device comprising

• a first reservoir (1) having a predetermined sub-ambient pressure and volume and a presealed opening (1a),
• a second reservoir (2) containing a predetermined volume of a fluid (M) and having a presealed opening (2a),
• a chamber (3) having an internal space (6) and an inlet (4) to the space (6) configured to be connected to the opening (2a) of the second reservoir (2) and an outlet (5) from the space (6) configured to be connected to the opening (1a) of the first reservoir (1),
• wherein the first and second reservoirs (1,2) are connected to each other such that the respective openings (1a,2a) are oriented to be simultaneously connectable to the inlet (4) and outlet (5) of the chamber (3), and
• wherein the first and second reservoirs (1,2) are arranged such that, upon connection with the inlet (4) and outlet (6) of the chamber (3), the opening (1a) of the first reservoir (1) is opened before the opening (2a) of the second reservoir (2) is opened.