Sample collection and transfer device

A method for individual biological sample collection and transfer, comprising;

• a) providing an integrated device, wherein the device comprises:
• a capillary channel disposed between an inner hydrophilic surface of a first layer and an inner hydrophilic surface of a second layer, wherein the first layer comprises a fluid inlet for receiving a sample fluid to the capillary channel, and the second layer comprises an outlet for allowing the fluid to flow out from the capillary channel, wherein the capillary channel has a width greater that a width of the outlet;
• and
• a third layer comprising a flow path therethrough, wherein the third layer is made of a pressure sensitive adhesive gasket and is disposed on an outer surface of the second layer at a determining position relative to the outlet, such that the outlet is in contact with the flow path of the third layer for transferring the sample fluid out from the integrated device;
• b) contacting the integrated device to a substrate comprising an absorbent material;
• c) applying an individual biological fluid sample to the capillary inlet of the integrated device, wherein the fluid sample is transported from the inlet to the outlet of the capillary;
• and
• d) transferring the fluid from the integrated device to the substrate through the flow path of the third layer;
• e) wherein the sample collection nd transfer is achieved at least in 5 seconds.