Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-CoA-derived products
AssigneesDanisco US Inc., The Goodyear Tire & Rubber Company
InventorsMarguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
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Abstract
The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.
Claims20
Recombinant cells capable of producing isoprene, wherein the cells:
- (i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide;
- (b) comprise a nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide;
- (ii) comprise one or more nucleic acids encoding one or more polypeptides of a mevalonate (MVA) pathway:
- and
- (iii) comprise a heterologous nucleic acid encoding a polypeptide having isoprene synthase activity, wherein culturing of the recombinant cells in a suitable media provides for the production of isoprene.
Recombinant cells capable of producing an isoprenoid precursor, wherein the cells:
- (i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide;
- and
- (b) comprise a nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide;
- and
- (ii) comprise one or more nucleic acids encoding one or more polypeptides of a MVA pathway, wherein culturing of the recombinant cells in a suitable media provides for the production of the isoprenoid precursor.
Recombinant cells capable of producing an isoprenoid, wherein the cells:
- (i) (a) have been modified such that the activity of a YfiQ polypeptide is modulated by decreasing, attenuating, or deleting the expression of a nucleic acid encoding the YfiQ polypeptide;
- and
- (b) comprise a nucleic acid encoding a CobB polypeptide, wherein the cells have been modified such that the activity of the CobB polypeptide is modulated by increasing the expression of the nucleic acid encoding the CobB polypeptide;
- (ii) comprise one or more nucleic acids encoding one or more polypeptides of a MVA pathway;
- and
- (iii) comprise a heterologous nucleic acid encoding a polyprenyl pyrophosphate synthase polypeptide, wherein culturing of the recombinant cells in a suitable media provides for production of the isoprenoid.
Dependencies are inferred from the claim text.