Gene-deleted variant strain of porcine pseudorabies virus, vaccine composition, method of making the same and use thereof

A vaccine composition, comprising:

• an immunizing amount of an antigen of an attenuated, variant strain of porcine pseudorabies virus;
• and a carrier, wherein the attenuated, variant strain of porcine pseudorabies virus comprises:
• a first attenuated porcine pseudorabies virus strain, wherein the first attenuated porcine pseudorabies virus strain comprises a porcine pseudorabies virus strain characterized by inactivation of gI/gE/11K/28K protein, wherein the first attenuated porcine pseudorabies virus strain is a variant strain of pseudorabies virus, wherein the gE protein has 95% or greater homology with SEQ ID NO:
• 5, and wherein the first attenuated strain of porcine pseudorabies virus strain comprises a gene variation resulting in inactivation of TK protein, wherein at least a portion of a TK gene is deleted from a first nucleotide sequence to generate a second nucleotide sequence, and wherein the second nucleotide sequence encodes for SEQ ID NO:
• 4.