US 10,504,711 B2 · Mass spectrometric method and MALDI-TOF mass spectrometer

Abstract

A number of standard methods exist for normalizing MALDI-TOF data, but they are not able to compensate adequately for the observed technical variability. The invention creates an improved normalization method for MALDI-TOF mass spectrometry data. This is achieved by an intensity profile normalization, where, by way of example, 1) Firstly, an intensity profile is formed for each individual spectrum, and this intensity profile describes the statistical distribution of the intensity values within different mass ranges; 2) Then an average reference profile is formed for an ensemble of spectra; 3) Finally, the individual spectra are transformed in such a way that their intensity profiles correspond to the reference profile.

Claims14

Dependent claims

A mass spectrometric method for the analysis of macromolecules from biological samples using a MALDI-TOF mass spectrometer, where the spectral intensities of the spectra from the mass spectrometry data are normalized by means of an intensity profile normalization, including the following steps:

(i) forming an intensity profile for each individual spectrum, which intensity profile describes the statistical distribution of the intensity values within different mass ranges;
(ii) forming an average reference profile for an ensemble of spectra;
and
(iii) transforming the individual spectra in such a way that their intensity profiles correspond to the reference profile.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

16/135,496Filed 19 Sep 2018

Priority

DE 10 2017 008 88522 Sep 2017

Published

US 2019/0096652 A128 Mar 2019

Granted

10 Dec 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

H01J 49/0036
Methods for using particle spectrometers: step by step routines describing the handling of the data generated during a measurement
G01N 33/6851
Methods of protein analysis involving mass spectrometry: methods of protein analysis involving laser desorption ionisation mass spectrometry
H01J 49/004
Particle spectrometers or separator tubes: combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
H01J 49/164
Using photoionisation, e.g. by laser: laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]

Mass spectrometric method and MALDI-TOF mass spectrometer

Life of this patent

Mass spectrometric method and MALDI-TOF mass spectrometer

Life of this patent

Abstract

Claims14