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US 10,503,573 B2Granted
Internal sizing/lane standard signal verification
AssigneeAnalogic Corporation
InventorChing Ming Lai
Life of this patent
Estimating the term…
Abstract
A method for verifying an ILS signal for DNA processing includes obtaining the ILS signal, determining acquisition times between peaks of the ILS signal, obtaining acquisition times between peaks in reference ILS information for the ILS signal, and verifying the ILS signal based on the ILS acquisition times and the reference ILS acquisitions times. An ILS signal processor includes a false peak remover that removes any false peaks in an ILS signal and a signal verifier that verifies the ILS signal includes only true peaks based on reference ILS information for the ILS signal.
Claims19
A method, comprising:
- receiving a sample at a sample processing apparatus;
- separating, at the sample processing apparatus, the sample into fragments;
- generating, with an optical reader of the sample processing apparatus, an output indicative of light sensed by the optical reader where the light is emitted by dyes in dye labeled and separated fragments of the sample in response to the optical reader directing a light beam of a predetermined wavelength range at the dye labeled and separated fragments;
- identifying, with a processor of the sample processing apparatus, a signal of the output that includes an Internal Lane Standard (ILS) signal, wherein the ILS signal has a first number of peaks and the first number of peaks are unequally spaced with respect to each other;
- determining, with the processor, acquisition times between peaks of the ILS signal;
- obtaining, with the processor, acquisition times between peaks in reference ILS information for the ILS signal, wherein the reference ILS information has a second number of peaks and the second number of peaks are unequally spaced with respect to each other, the second number of peaks is the same as the first number of peaks, and a spacing of the peaks in the ILS signal is different than a spacing in the reference ILS information;
- determining, with the processor, ratios of the ILS signal acquisition times to the reference ILS information acquisition times;
- verifying, with the processor, that the ILS signal includes only true peaks based on the ratios;
- translating, at the sample processing apparatus, acquisition times of the ILS signal into fragment sizes based on a verified ILS signal;
- and
- identifying DNA fragments based on the fragment sizes.
8 more elements
A method, comprising:
- receiving a sample at a sample processing apparatus;
- separating, at the sample processing apparatus, the sample into fragments;
- generating, with an optical reader of the sample processing apparatus, an output indicative of light sensed by the optical reader where the light is emitted by dyes in dye labeled and separated fragments of the sample in response to the optical reader directing a light beam of a predetermined wavelength range at the dye labeled and separated fragments;
- identifying, with a processor of the sample processing apparatus, a signal of the output that includes an Internal Lane Standard (ILS) signal;
- determining, with the processor, a number of peaks in the ILS signal, wherein the ILS signal has a first number of peaks and the first number of peaks includes at least one false peak;
- obtaining, with the processor, a number of peaks in reference ILS information, wherein the reference ILS information has a second number of peaks, and the first number of peaks is greater than the second number of peaks;
- determining, with the processor, the ILS signal includes one or more false peaks in response to the first number of peaks in the ILS signal being greater than the second number of peaks from the reference ILS information;
- determining, with the processor, acquisition times between peaks of the ILS signal;
- obtaining, with the processor, acquisition times between peaks in the reference ILS information;
- identifying the one or more false peaks based on ratios of the ILS signal acquisition times to the reference ILS information acquisition times;
- removing, with the processor, the one or more false peaks from the ILS signal;
- translating, at the sample processing apparatus, the acquisition times of the ILS signal into fragment sizes based on the ILS signal with the removed one or more false peaks;
- and
- identifying DNA fragments based on the fragment sizes.
11 more elements
The method of 9, further comprising:
- determining acquisition times between the peaks of the ILS signal;
- obtaining acquisition times between the peaks in reference ILS information for the ILS signal;
- determining ratios of the ILS signal acquisition times to the reference ILS information acquisition times;
- and
- verifying that the ILS signal includes only true peaks based on the ratios.
2 more elements
A method, comprising:
- receiving a sample at a sample processing apparatus;
- separating, at the sample processing apparatus, the sample into fragments;
- generating, with an optical reader of the sample processing apparatus, an output indicative of light sensed by the optical reader where the light is emitted by dyes in dye labeled and separated fragments of the sample in response to the optical reader directing a light beam of a predetermined wavelength range at the dye labeled and separated fragments;
- identifying, with a processor of the sample processing apparatus, a signal of the output that includes an Internal Lane Standard (ILS) signal;
- wherein each of the ILS signal and the reference ILS information includes equally spaced peaks having at least first and second amplitudes, and a number of peaks having a first amplitude is less than a number of the peaks having a second amplitude, wherein the first amplitude is greater than or less than the second amplitude;
- verifying, with the processor, the peaks with the first amplitude before and independent of the peaks with the second amplitude or verifying the peaks with the second amplitude before and independent of the peaks with the first amplitude;
- translating, at the sample processing apparatus, acquisition times of the ILS signal into fragment sizes based on a verified ILS signal;
- and
- identifying DNA fragments based on the fragment sizes.
6 more elements
The method of 16, where the first amplitude is greater than the second amplitude and the peaks with the first amplitude are verified first, further comprising:
- selecting the peaks with the second amplitude based on the verified peaks with the first amplitude;
- and
- verifying the peaks with the second amplitude between successive peaks with the first amplitude.
The method of 16, where the second amplitude is greater than the first amplitude and the peaks with the second amplitude are verified first, further comprising:
- selecting the peaks with the first amplitude based on the verified peaks with the second amplitude;
- and
- verifying the peaks with the first amplitude peaks between successive peaks with the second amplitude.
The method of 12, further comprising:
- determining acquisition times between the peaks of the ILS signal;
- obtaining acquisition times between the peaks in reference ILS information for the ILS signal;
- determining ratios of the ILS signal acquisition times to the reference ILS information acquisition times;
- and
- verifying that the ILS signal includes only true peaks based on the ratios.
2 more elements
Dependencies are inferred from the claim text.