Method for detecting lung squamous cell carcinoma

A method of detecting desmoglein 3 content in a blood sample from a subject, the method comprising the steps of:

• (1) measuring desmoglein 3 content in the blood sample collected from the subject;
• and
• (2) comparing the desmoglein 3 content measured in step (1) with the desmoglein 3 content in a blood sample collected from a healthy individual;
• and
• (3) setting a cut-off value for desmoglein 3 content such that a false positive ratio is 30% or less,
• wherein said measuring in step (1) is performed by a sandwich immunoassay, and
• wherein said sandwich immunoassay uses:
• as an immobilized antibody, a DF366m antibody being a complete antibody comprising an H chain represented by the amino acid sequence shown in SEQ ID NO:
• 2 and an L chain represented by SEQ ID NO:
• 3, or an antibody comprising an H chain having the amino acid sequence shown in SEQ ID NO:
• 10 as VH and an L chain having the amino acid sequence shown in SEQ ID NO:
• 11 as VL;
• said DF366m antibody having HCDR1 represented by the amino acid sequence shown in SEQ ID NO:4, HCDR2 represented by the amino acid sequence shown in SEQ ID NO:5, HCDR3 represented by the amino acid sequence shown in SEQ ID NO:6, LCDR1 represented by the amino acid sequence shown in SEQ ID NO:7, LCDR2 represented by the amino acid sequence shown in SEQ ID NO:8 and LCDR3 represented by the amino acid sequence shown in SEQ ID NO:9;
• and,
• as a detection antibody an MAB1720 antibody or a DF151 antibody being a complete antibody comprising an H chain represented by the amino acid sequence shown in SEQ ID NO:15 and an L chain represented by SEQ ID NO:16, or an antibody comprising an H chain having the amino acid sequence shown in SEQ ID NO:23 as VH and an L chain having the amino acid sequence shown in SEQ ID NO:24 as VL;
• said DF151 antibody having HCDR1 represented by the amino acid sequence shown in SEQ ID NO:17, HCDR2 represented by the amino acid sequence shown in SEQ ID NO:18, HCDR3 represented by the amino acid sequence shown in SEQ ID NO:19, LCDR1 represented by the amino acid sequence shown in SEQ ID NO:20, LCDR2 represented by the amino acid sequence shown in SEQ ID NO:21 and LCDR3 represented by the amino acid sequence shown in SEQ ID NO:22,
• wherein said sandwich immunoassay is surface plasmon-field enhanced fluorescence spectroscopy and has a signal to noise ratio of 60.8 to 140 at a desmoglein 3 concentration of 1,000 pg/ml.