US 10,502,732 B2 · Detection of pathogens using unmodified metal nanoparticles

Abstract

The present disclosure relates to a method for the direct detection of pathogen in a sample using unmodified metallic nanoparticles, such as gold nanoparticles. The method may employ colorimetric detection. The combination of unmodified metallic nanoparticles and colorimetric detection provides a method that is simple, rapid, and economical compared to prior art methods that require modified nanoparticles or expensive detection equipment. The method does not require labeling of the target pathogen and is capable of detecting a broad spectrum of pathogens.

Claims16

Dependent claims

A method of directly detecting a pathogen in a sample comprising:

a) obtaining a sample suspected of containing a pathogen;
b) contacting the sample with a plurality of unmodified metallic nanoparticles under conditions suitable to permit direct association of the nanoparticles with a surface of the pathogen, if present,
wherein the surface comprises teichoic acids, lipids, polysaccharides and/or proteins, and wherein the pathogen is not modified prior to the contacting;
and
c) assessing the direct association of the nanoparticles with the surface of the pathogen to determine whether the pathogen is present or absent,
wherein assessing the association of the nanoparticles with the pathogen comprises assessing a colorimetric property of the sample.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/893,297Filed 23 May 2014 · us provisional application

Priority

24 May 2013

Published

US 2016/0123967 A15 May 2016

Granted

10 Dec 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification11 codes

G01N 33/54346
The carrier being characterised by its particulate form: nanoparticles
C12Q 1/04
Involving viable microorganisms: determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides;…
G01N 33/56911
For microorganisms, e.g. protozoa, bacteria, viruses: bacteria
B82Y 30/00
Nanotechnology for materials or surface science

Detection of pathogens using unmodified metal nanoparticles

Life of this patent

Detection of pathogens using unmodified metal nanoparticles

Life of this patent

Abstract

Claims16