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US 10,502,685 B2Granted
Method for urine sample analysis, reagent for urine sample analysis, and reagent kit for urine sample analysis
AssigneeSysmex Corporation
InventorAkinori Kawai
Life of this patent
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Abstract
The present invention relates to a method for analyzing urine sample, a reagent for analysis of urine sample and a reagent kit for analysis of urine sample, which are for detecting at least casts and erythrocytes as urinary particles.
Claims14
A method for analyzing a urine sample, comprising the steps of:
- staining casts and erythrocytes in the urine sample with a fluorescent dye by mixing the urine sample with a first reagent containing the fluorescent dye, the fluorescent dye being at least one selected from the group consisting of 3,3′-diethyloxacarbocyanine iodide (DiOC2(3)), 3,3-dipropyloxacarbocyanine iodide (DiOC3(3)), 3,3′-dibutyloxacyanine iodide (DiOC4(3)) and 3,3-dipentyloxacarbocyanine iodide (DiOC5(3)),
- dispersing aggregates of impurities in the urine sample with dodecyltrimethylammonium bromide by mixing a mixture of the urine sample and the first reagent with a second reagent containing dodecyltrimethylammonium bromide and obtaining a measurement sample,
- obtaining scattered light information and fluorescence information by irradiating urinary particles contained in the measurement sample with light, and
- detecting at least the casts and the erythrocytes as the urinary particles in the measurement sample based on the obtained scattered light information and fluorescence information.
1 more element
Dependencies are inferred from the claim text.