Glucose sensors and methods of use thereof
AssigneeInternational Park of Creativity
InventorRaul Cuero Rengifo
Life of this patent
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Abstract
Described herein are glucose and insulin sensors. The sensors are composed of host cells with DNA specifically designed to produce fluorescence when the cells come into contact with glucose and/or insulin in the sample. Once the fluorescence has been quantified, it can be correlated with the amount of glucose and/or insulin present in the sample.
Claims10
A host cell comprising a DNA construct comprising (a) a gene that expresses an alcohol dehydrogenase 1 (ADH1) promoter having at least 95% homology to SEQ ID NOS 1 or 30-32, (b) a gene that expresses snf3 protein having at least 95% homology to SEQ ID NOS 2 or 20-29, and (c) a ribosomal switch.
A host cell comprising a DNA construct comprising (a) a gene that expresses an alcohol dehydrogenase 1 (ADH1) promoter having at least 95% homology to SEQ ID NOS 1 or 30-32 and (b) a gene that expresses snf3 protein having at least 95% homology to SEQ ID NOS 2 or 20-29, wherein the construct comprises a vector, wherein the vector is pWLNEO, pSV2CAT, pOG44, PXT1, pSG, pSVK3, pBSK, pBR322, pYES, PBSKII, or a pUC vector.
Dependencies are inferred from the claim text.