US 10,501,813 B2Granted

Enzyme preparation containing thermostable DNA polymerase, method for producing same, and method for detecting subject organism to be detected

AssigneesHokkaido Mitsui Chemicals Inc., National University Corporation University of Toyama

InventorsHomare Tabata, Hiroshi Minami, Hideki Niimi, Isao Kitajima, Tomohiro Ueno, Shiroh Hayashi, Masashi Mori

Life of this patent

Estimating the term…

Loading patent…

Abstract

Disclosed is a thermostable DNA polymerase preparation which can illimitably reduce the risk of false positivity in the detection of a subject microorganism utilizing a gene amplification reaction and therefore enables the selective amplification of DNA for detecting the subject microorganism even when the amount of the subject microorganism is small and therefore the amount of DNA collected therefrom is extremely small, and can be produced at a reduced cost. Also disclosed is a method for quantifying or quantifying/identifying a subject organism to be detected rapidly, conveniently and with high sensitivity using the preparation of the present invention.

Claims5

Dependent claims

A production method for a thermostable DNA polymerase preparation, comprising the steps of:

(1) culturing transformant eukaryotic cells selected from the group consisting of fungi and plant cells carrying a gene encoding the thermostable DNA polymerase,
(2) obtaining an extract containing the thermostable DNA polymerase from the cultured transformant eukaryotic cells, followed by subjecting the extract to heat treatment, or
subjecting the cultured transformant eukaryotic cells to heat treatment, followed by obtaining an extract containing the thermostable DNA polymerase from the heat-treated transformant eukaryotic cells,
wherein the gene encoding the thermostable DNA polymerase comprises DNA consisting of the base sequence of SEQ ID NO:1.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 15/688,473Filed 28 Aug 2017 · division
Priority: JP 2009-00655615 Jan 2009
Published: US 2018/0057860 A11 Mar 2018
Granted: 10 Dec 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification11 codes

C12Q 1/689
For detection or identification of organisms: for bacteria
C12N 9/1252
Nucleotidyltransferases: dNA-directed DNA polymerase , i.e. DNA replicase
C12P 19/34
Nucleotides: polynucleotides, e.g. nucleic acids, oligoribonucleotides
C12Q 1/68
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…

Family

PCT/JP2010/050443Parent of this division · filed 15 Jan 2010
Application14/969,252Parent of this division · filed 15 Dec 2015
US 9,243,272 B2Parent of this division · filed 26 Jan 2016
US 2018/0057860 A1Pre-grant publication · published 1 Mar 2018
Application13/144,175Parent of this division
US 2012/0094296 A1Published application
US 2016/0257999 A1Published application
US 10,501,813 B2This patent

From the US filing history. Foreign family members aren’t listed.

Timeline

Citations

Open elsewhere

Building an agent or pulling many patents? Use the API or MCP server instead of scraping pages. Get an API key