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US 10,501,812 B2Granted
Compositions, kits and related methods for the detection and/or monitoring of Listeria
AssigneeGen-Probe Incorporated
InventorsMichael R. Reshatoff, Kristin W. Livezey, James J. Hogan
Life of this patent
Estimating the term…
Abstract
Provided are compositions, kits, and methods for the identification of Listeria. In certain aspects and embodiments, the compositions, kits, and methods may provide improvements in relation to specificity, sensitivity, and speed of detection.
Claims20
A method for detecting Listeria in a sample, the method comprising:
- performing a nucleic acid amplification assay using a set of oligonucleotides comprising a T7 provider oligonucleotide and a primer oligonucleotide, wherein the T7 provider oligonucleotide and the primer oligonucleotide target sequences in a region of Listeria nucleic acid corresponding to nucleotides 1180-1370 of the 16S rRNA of E. coli, represented by SEQ ID NO:113, and wherein the T7 provider oligonucleotide and the primer oligonucleotide used in said amplification assay target opposite strands of the Listeria nucleic acid sequence to be amplified, and
- detecting the presence or absence of amplification product, wherein the presence of the amplification product indicates the presence of Listeria in the sample.
Dependencies are inferred from the claim text.