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US 10,501,811 B2Granted
Method for sorting tissue cells
AssigneeKyoto University
InventorsYoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
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Abstract
An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.
Claims3
A method for producing insulin-producing cells, comprising:
- (a) producing, from a pluripotent stem cell, a cell population containing insulin-producing cells;
- (b) introducing, into the cell population of the step (a), an miRNA-responsive mRNA consisting of sequences comprising (i) a nucleic acid recognized specifically by miR-375 and (ii) a nucleic acid corresponding to a coding region of a first marker gene, wherein the miRNA-responsive mRNA is introduced into the cell population in a form of a synthetic RNA molecule;
- and
- (c) sorting a cell having a low level of translation of the first marker gene of the step (b).
1 more element
Dependencies are inferred from the claim text.