Isoforms of GATA6 and NKX2-1 as markers for diagnosis and therapy of cancer and as targets for anti-cancer therapy

A method of assessing a lung cell sample or a breath condensate from a subject, said method comprising

• a) measuring in the lung cell sample or breath condensate of said subject the amount of specific transcription factor isoforms by performing detection assay to specifically detect said specific transcription factor isoforms, wherein said specific transcription isoforms are
• i) the GATA6 Em isoform comprising the nucleic acid sequence of SEQ ID No:
• 1 or the GATA6 Em isoform comprising a nucleic acid sequence of SEQ ID NO:1 with at least one of the SNPs selected from
• G at position 293 is substituted with A;
• G at position 320 is substituted with C;
• C at position 327 is substituted with G;
• C at position 339 is substituted with G;
• G at position 430 is substituted with U;
• position 462 is inserted with U;
• A at position 480 is substituted with U;
• C at position 759 is substituted with U;
• C at position 1128 is substituted with G;
• C at position 1256 is substituted with A;
• G at position 1304 is substituted with A;
• C at position 1589 is substituted with U;
• U at position 1597 is substituted with A;
• A at position 1627 is substituted with G;
• C at position 1651 is substituted with U;
• G at position 1652 is substituted with A;
• A at position 1803 is substituted with G;
• U at position 1844 is substituted with C;
• U at position 1849 is substituted with C;
• A at position 1879 is substituted with G;
• A at position 1882 is substituted with G;
• U at position 1911 is substituted with G;
• C at position 1940 is substituted with G;
• A at position 1949 is substituted with G;
• U at position 1982 is substituted with C;
• G at position 2000 is substituted with C;
• C at position 2002 is substituted with U;
• G at position 2008 is substituted with C;
• C at position 2026 is substituted with U;
• G at position 2031 is substituted with U;
• C at position 2106 is substituted with U;
• G at position 2137 is substituted with A;
• A at position 2142 is substituted with G
• C at position 2163 is substituted with U;
• C at position 2294 is substituted with U;
• A at position 2390 is substituted with G;
• U at position 2391 is substituted with A;
• A at position 2627 is substituted with G;
• A at position 3102 is substituted with G;
• C at position 3240 is substituted with U;
• A at position 3290 is substituted with G;
• C at position 3358 is substituted with U;
• A at position 3366 is substituted with U;
• C at position 3578 is substituted with U;
• position 3632 is inserted with C;
• C at position 3646 is substituted with U;
• A at position 3670 is substituted with G;
• and
• C at position 3690 is substituted with U;
• and
• ii) the NKX2-1 Em isoform comprising the nucleic acid sequence of SEQ ID No:
• 2 or the NKX2-1 Em isoform comprising a nucleic acid sequence of SEQ ID NO:
• 2 with at least one of the SNPs selected from
• position 305 is inserted with A;
• position 304 is inserted with AA;
• G at position 420 is substituted with A;
• C at position 425 is substituted with U;
• G at position 439 is substituted with U;
• C at position 441 is substituted with A;
• C at position 450 is substituted with U;
• C at position 486 is substituted with U;
• G at position 781 is substituted with U;
• C at position 785 is substituted with U;
• A at position 825 is substituted with C;
• G at position 950 is substituted with U;
• G at position 1169 is substituted with A;
• G at position 1305 is substituted with A;
• G at position 1344 is substituted with U;
• G at position 1448 is substituted with A;
• position 1640 is inserted with U;
• position 1641 is inserted with GU;
• position 1643 is inserted with >6 bp;
• position 1673 is inserted with U;
• position 1678 is inserted with U;
• position 1748 is inserted with C;
• position 1750 is inserted with C;
• position 1916 is inserted with A;
• position 1917 is inserted with A;
• C at position 1934 is substituted with G or U;
• and
• C at position 2099 is substituted with G.