PCR amplification methods for detecting and quantifying sulfate-reducing bacteria in oilfield fluids
AssigneeBaker Hughes, A Ge Company, LLC
InventorCrystal Lee
Life of this patent
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Abstract
At least one nucleic acid from a sulfate-reducing bacteria (SRB) may be extracted from an oilfield fluid and may be amplified by a PCR amplification method in the presence of at least one primer to form an amplification product. The primer(s) may be or include a sequence including, but not necessarily limited to, SEQ ID NO: 20, SEQ ID NO: 21, and mixtures thereof. The amplification product may be hybridized with a probe specific for a fragment of an alpha subunit of an APS gene, and a presence of hybridization and a degree of hybridization may be detected.
Claims15
A method of decreasing sulfate-reducing bacteria (SRB) in oilfield fluids comprising:
- altering an amount of a microbial agent within an oilfield fluid to form an altered oilfield fluid based on an amount of at least one SRB within an oilfield fluid;
- wherein the amount of the at least one SRB is determined by:
- amplifying at least one nucleic acid of the at least one SRB in the presence of at least one primer to form an amplification product;
- wherein the at least one nucleic acid is extracted from the oilfield fluid prior to amplifying the at least one nucleic acid;
- wherein the at least one primer comprises a sequence selected from the group consisting of SEQ ID NO.20, SEQ ID NO:21, and mixtures thereof;
- hybridizing the amplification product with a probe specific for a fragment of an alpha subunit of an Adenylylsulfate Reductase gene;
- and
- detecting a presence of hybridization and a degree of hybridization, wherein the presence of hybridization indicates the presence of the at least one SRB, and wherein the degree of hybridization enumerates the at least one SRB;
- and
- decreasing the amount of SRB by killing and/or deactivating the SRB wherein the altered oilfield fluid comprises a decreased amount of SRB as compared to the oilfield fluid.
A method of decreasing sulfate-reducing bacteria (SRB) in oilfield fluids comprising:
- altering an amount of a microbial agent within an oilfield fluid based on an amount of at least one SRB within the oilfield fluid to form an altered oilfield fluid;
- wherein the oilfield fluid is selected from the group consisting of oilfield water, a production fluid, a fracturing fluid, a drilling fluid, a completion fluid, a workover fluid, a packer fluid, a gas fluid, a crude oil, and mixtures thereof;
- wherein the amount of the at least one SRB is determined by:
- amplifying at least one nucleic acid of the at least one SRB in the presence of at least one primer to form an amplification product;
- wherein the at least one nucleic acid is extracted from the oilfield fluid prior to amplifying the at least one nucleic acid;
- wherein the at least one primer comprises a sequence selected from the group consisting of SEQ ID NO.20, SEQ ID NO:21, and mixtures thereof;
- hybridizing the amplification product with a probe specific for a fragment of an alpha subunit of an Adenylylsulfate Reductase gene;
- and
- detecting a presence of hybridization and a degree of hybridization;
- wherein the presence of hybridization indicates the presence of the at least one SRB;
- and wherein the degree of hybridization enumerates the at least one SRB;
- and
- decreasing the amount of SRB by killing and/or deactivating the SRB.
A method of determining an amount of sulfate-reducing bacteria (SRB) within an oilfield fluid comprising:
- amplifying at least one nucleic acid of at least one SRB in the presence of at least one primer to form an amplification product;
- wherein the amplifying occurs by a PCR amplification method wherein the at least one nucleic acid is extracted from the oilfield fluid prior to amplifying the at least one nucleic acid;
- wherein the at least one primer comprises a selected from the group consisting of SEQ ID NO.20, SEQ ID NO:21, and mixtures thereof;
- hybridizing the amplification product with a probe specific for a fragment of an alpha subunit of an Adenylylsulfate Reductase gene;
- and
- detecting a presence of hybridization and a degree of hybridization;
- wherein the presence of hybridization indicates the presence of the at least one SRB;
- and wherein the degree of hybridization enumerates the at least one SRB;
- and
- determining an amount of SRB in the oilfield fluid.
A PCR amplification method comprising:
- amplifying at least one nucleic acid of at least one sulfate-reducing bacteria (SRB) in the presence of at least one primer to form an amplification product;
- wherein the at least one SRB is extracted from an oilfield fluid prior to amplifying the at least one nucleic acid;
- wherein the at least one primer comprises a sequence selected from the group consisting of SEQ ID NO.20, SEQ ID NO:21, and mixtures thereof.
Dependencies are inferred from the claim text.