Microfluidic process for treating and analysing a solution containing a biological material and corresponding microfluidic circuit

A microfluidic process for partitioning a solution, said microfluidic process comprising the steps of:

• a. providing a microfluidic circuit, wherein microchannels suitable for containing fluids are defined, said circuit comprising at least one device for forming a plurality of droplets of a solution in a carrier fluid, and at least one storage zone, wherein:
• said at least one device for forming droplets further comprises said microchannels, wherein each of said microchannels comprise wall portions that diverge to detach a droplet of said solution under the effect of surface tension of said solution,
• said at least one storage zone comprises a rectangular-shaped step on at least one of its bounds,
• said at least one device for forming droplets opens onto said rectangular-shaped step of said at least one storage zone, and
• said at least one storage zone is a zone where the plurality of droplets is held after formation in the microfluidic circuit but cannot come out without external intervention, regardless of the carrier fluid flow;
• b. filling the microfluidic circuit with the carrier fluid;
• and
• c. flowing the solution in the carrier fluid through the microchannels of the microfluidic circuit,
• thereby generating a plurality of droplets of said solution.