System and methods for calibrating binding dyes
AssigneeLife Technologies Corporation
InventorsYong Chu, Jacob Freudenthal, Jeffrey Marks
Life of this patent
Estimating the term…
Abstract
Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. The sample holder can comprise a plurality of reaction sites and more than one dye type, with each dye occupying more than one reaction site. The method can further comprise identifying a peak channel for each dye on the sample holder, normalizing each channel to the peak channel for each dye, and producing a dye matrix that can comprise a set of dye reference values.
Claims20
A computer-implemented method for calibrating a fluorescent dye, the method comprising:
- imaging a sample holder, loaded into an instrument, at more than one channel, the sample holder comprising a plurality of reaction sites and a plurality of dyes comprising more than one dye type, each dye occupying more than one reaction site, wherein the plurality of dyes are distributed in a checkerboard pattern among the reaction sites;
- identifying a peak channel for each dye on the sample holder;
- normalizing each channel to the peak channel for each dye;
- and
- producing a dye matrix comprising a set of dye reference values.
A non-transitory computer-readable storage medium encoded with instructions, executable by a processor, for calibrating a fluorescent dye, the instructions comprising instructions for:
- imaging a sample holder, loaded into an instrument, at more than one channel, the sample holder comprising a plurality of reaction sites and a plurality of dyes comprising more than one dye type, each dye occupying more than one reaction site, wherein the plurality of dyes are distributed in a checkerboard pattern among the reaction sites;
- identifying a peak channel for each dye on the sample holder;
- normalizing each channel to the peak channel for each dye;
- and
- producing a dye matrix comprising a set of dye reference values.
A system for calibrating a fluorescent dye, the system comprising:
- a processor;
- and
- a memory encoded with instructions, executable by the processor, the instructions for:
- imaging a sample holder, loaded into an instrument, at more than one channel, the sample holder comprising a plurality of reaction sites and a plurality of dyes comprising more than one dye type, each dye occupying more than one reaction site, wherein the plurality of dyes are distributed in a checkerboard pattern among the reaction sites;
- identifying a peak channel for each dye on the sample holder;
- normalizing each channel to the peak channel for each dye;
- and
- producing a dye matrix comprising a set of dye reference values.
Dependencies are inferred from the claim text.