Cell-free synthesis of DNA by strand displacement
AssigneeTouchlight IP Limited
InventorsNeil Porter, Paul Rothwell, Jonathan Extance
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Abstract
The present invention relates to an improved process for synthesis of DNA, RNA, proteins and like molecules, in particular cell-free enzymatic synthesis of DNA, preferably in large scale. The present invention relates to the synthesis of DNA using strand-displacement replication and the addition of nucleotides to the reaction mixture is controlled, thus controlling yield. The reaction mixture contains a starting amount of nucleotides, polymerase and DNA template, to which further nucleotides are supplied in a controlled manner.
Claims31
A cell-free process for synthesising DNA comprising contacting a DNA template with at least one polymerase in the presence of nucleotides to form a reaction mixture, wherein the DNA template is amplified by strand displacement replication, and wherein further nucleotides are supplied to the reaction mixture continuously or at intervals during the process.
A cell-free process for synthesising DNA comprising contacting a DNA template with at least one polymerase in the presence of nucleotides and at least one primer to form a reaction mixture, wherein the DNA template is amplified by strand displacement replication, and wherein further nucleotides are supplied to the reaction mixture continuously or at intervals during the process.
Dependencies are inferred from the claim text.