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US 10,501,780 B2Granted
Compositions for in situ nucleic acid analysis
AssigneeApplied Biosystems, LLC
InventorsRichard A. Fekete, Annalee Nguyen
Life of this patent
Estimating the term…
Abstract
Sample preparation processes for in situ RNA or DNA analysis, methods and compositions therefor are provided. Processes provided herein allow DNA or RNA analysis to be carried out in the same tube or on an aliquot of the prepared sample without centrifugation or extraction. The preparation process can be carried out at room temperature in as little as seven minutes and is amenable to high throughput processing using manual or robotic platforms.
Claims20
A composition comprising:
- a polypeptide having deoxyribonuclease activity and a surfactant that substantially lacks fluorescence between 300 nm and 750 nm when in use for in situ analysis of nucleic acid, wherein the composition is substantially free of a cation chelator.
A composition comprising:
- a polypeptide having deoxyribonuclease activity and a surfactant that substantially lacks fluorescence between 300 nm and 750 nm when in use for in situ analysis of nucleic acid, wherein the composition is substantially free of a cation chelator, wherein the surfactant comprises octylphenol ethoxylate having an average of 7.5 ethoxylate groups, and wherein the composition comprises less than 1 mM cation chelator.
A composition comprising:
- a polypeptide having deoxyribonuclease activity and a surfactant that substantially lacks fluorescence between 300 nm and 750 nm when in use for in situ analysis of nucleic acid, wherein the composition is substantially free of a cation chelator, wherein the surfactant comprises octylphenol ethoxylate having an average of 9.5 ethoxylate groups, and wherein the composition comprises less than 1 mM cation chelator.
Dependencies are inferred from the claim text.