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US 10,501,774 B2Granted
Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates
AssigneePromega Corporation
InventorsMichael P. Valley, James J. Cali, Brock Binkowski, Christopher Todd Eggers, Keith V. Wood
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Abstract
Methods, kits and compositions containing a mixture of D-luciferin and L-luciferin for light generation with luciferase are disclosed that have improved stability when stored over time. The mixture of D-luciferin and L-luciferin can be used to detect the presence or amount of ATP or of luciferase in a sample.
Claims22
A luciferase reaction composition comprising:
- a luciferase;
- and
- a combination of D-luciferin and L-luciferin present in the composition in a ratio from about 0.25:1 to about 4:1 of D-luciferin to L-luciferin;
- wherein the composition is substantially free of adenosine triphosphate (ATP);
- and
- wherein the composition retains at least 50% luciferase activity after being stored for at least about 1 day at a temperature from about 0° C. to about 35° C.
3 more elements
Dependencies are inferred from the claim text.