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US 10,501,772 B2Granted
Methods for rapid antimicrobial susceptibility testing
AssigneeSelux Diagnostics, Inc.
InventorsEric Stern, Aleksandar Vacic, Benjamin Spears, Alec Flyer, Kelly Flentie
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Abstract
The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.
Claims45
A method for determining antimicrobial susceptibility of microorganisms comprising:
- incubating a liquid suspension of microorganisms in the presence of an antimicrobial under conditions that promote growth of the microorganisms,
- adding a signaling agent that binds to a surface of the microorganisms;
- separating the microorganisms bound by the signaling agent from unbound signaling agent;
- and
- measuring signal levels associated with the microorganisms as compared to one or more controls, thereby measuring the antimicrobial susceptibility of the microorganisms;
- wherein the signaling agent comprises a linker group L, and an amplifier group 104 comprises an Europium coordination complex;
- and wherein,
- L forms a covalent bond to the amplifier group 104;
- or
- L forms one or more non-covalent interactions with an amplifier group 104.
7 more elements
A method for determining antimicrobial susceptibility of microorganisms comprising:
- incubating a liquid suspension of microorganisms in a cartridge comprising a plurality of chambers, each chamber containing one or more antimicrobials, under conditions that promote growth of the microorganisms;
- adding a signaling agent to the plurality of chambers, wherein the signaling agent binds to a surface of the microorganisms
- removing unbound signaling agent;
- and
- measuring signaling levels in the plurality of chambers as compared to one or more controls, thereby determining the susceptibility of microorganisms to the one or more antimicrobials;
- wherein the signaling agent comprises a linker group L, and an amplifier group 104 comprises an Europium coordination complex;
- and wherein,
- L forms a covalent bond to the amplifier group 104;
- or
- L forms one or more non-covalent interactions with an amplifier group 104.
7 more elements
Dependencies are inferred from the claim text.