US 10,501,769 B2 · Method for the production of a glycosylated immunoglobulin

Abstract

Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80% of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture.

Claims41

Dependent claims

A method for the production of an immunoglobulin comprising

a) cultivating a Chinese Hamster Ovary (CHO) cell comprising a nucleic acid encoding the immunoglobulin in a cultivation medium with restricted glucose feeding wherein the amount of glucose available in the cultivation medium per time unit is kept constant in all time units in which restricted glucose feeding is performed and the degree of glucose limitation (DGL) is limited to a single constant value in a range between 0.5 and 0.1, wherein feeding is started once the amount of glucose present in the cultivation medium has dropped to or below a preset value in the cultivation and wherein said cultivation is a fed-batch cultivating, and
b) recovering the immunoglobulin.

Dependencies are inferred from the claim text.

Facts

Application

14/844,570Filed 3 Sep 2015 · continuation

Priority

EP 0901345526 Oct 2009

Published

US 2016/0186228 A130 Jun 2016

Granted

10 Dec 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

C12P 21/005
Preparation of peptides or proteins: glycopeptides, glycoproteins
C07K 16/2866
Against receptors, cell surface antigens or cell surface determinants: against receptors for cytokines, lymphokines, interferons
C07K 2317/14
Characterized by their source of isolation or production: specific host cells or culture conditions, e.g. components, pH or temperature
C07K 2317/24
Characterized by taxonomic origin: containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered

Method for the production of a glycosylated immunoglobulin

Life of this patent

Method for the production of a glycosylated immunoglobulin

Life of this patent

Abstract

Claims41