Method of producing RNA from circular DNA and corresponding template DNA

A method of producing a linear target RNA comprising the steps of:

• a) providing a circular DNA as template DNA comprising the following sequence elements from 5′ to 3′:
• i. an RNA polymerase promoter sequence operably linked to
• ii. a sequence encoding said target RNA operably linked to
• iii. a sequence encoding a self-cleaving ribozyme, wherein said self-cleaving ribozyme cleaves close to or at its 5′ end, operably linked to
• iv. an RNA polymerase terminator sequence element;
• and
• b) in vitro transcribing said template DNA to obtain said target RNA;
• c) purifying said target RNA by at least one purification step in order to obtain purified target RNA;
• wherein said method does not comprise a step of linearizing said circular DNA provided in step a),
• wherein said RNA polymerase terminator sequence element comprises at least two Class II termination sequences.