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US 10,501,767 B2Granted
Polynucleotide modification methods
AssigneeOxford Nanopore Technologies PLC
InventorsDavid Jackson Stoddart, James White
Life of this patent
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Abstract
The invention relates to a method for modifying a template double stranded polynucleotide, especially for characterization using nanopore sequencing. The method produces from the template a plurality of modified double stranded polynucleotides. These modified polynucleotides can then be characterized.
Claims14
A method for modifying a template double stranded polynucleotide, comprising:
- (a) contacting a double stranded template polynucleotide with a MuA transposase and a population of double stranded MuA substrates each comprising at least one overhang of universal nucleotides, such that the transposase fragments the template polynucleotide into two or more double-stranded fragments, and ligates a substrate to an end of each of the double stranded fragments, thereby producing a plurality of fragment/substrate constructs;
- and
- (b) ligating the overhangs to the fragments in the constructs and thereby producing a plurality of modified double stranded polynucleotides.
Dependencies are inferred from the claim text.