Recombinant baculovirus and method for using the same for preparing recombinant adeno-associated virus vector
AssigneeWuhan Institute of Physics and Mathematics, Chinese Academy of Sciences
InventorsYang Wu, Fuqiang Xu, Xiaobin He
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Abstract
A recombinant baculovirus, including: an adeno-associated virus Rep gene, an adeno-associated virus Cap gene, and an recombinant adeno-associated virus genome ITR-GOI (gene of interest) flanked by rAAV inverted terminal repeats (ITR). The ITR-GOI includes a 5′ terminal nucleic acid fragment and a 3′ terminal nucleic acid fragment. The ITR-GOI is linked to an expression cassette of the Cap gene and an expression cassette of the Rep gene through the 5′ terminal nucleic acid fragment and the 3′ terminal nucleic acid fragment, respectively.
Claims12
A recombinant baculovirus, comprising:
- an adeno-associated virus (AAV) Rep gene, an AAV Cap gene, and a recombinant adeno-associated virus (rAAV) genome ITR-GOI (gene of interest) flanked by AAV inverted terminal repeats (ITR);
- wherein:
- the ITR-GOI comprises a 5′ terminal nucleic acid fragment and a 3′ terminal nucleic acid fragment;
- and
- the ITR-GOI is linked to an expression cassette of the Cap gene and an expression cassette of the Rep gene through the 5′ terminal nucleic acid fragment and the 3′ terminal nucleic acid fragment, respectively.
Dependencies are inferred from the claim text.