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US 10,501,748 B2Granted
Methods and compositions for integration of an exogenous sequence within the genome of plants
AssigneesDow AgroSciences LLC, Sangamo Therapeutics, Inc.
InventorsW. Michael Ainley, Dmitry Y. Guschin, Matthew Hayden, Daniel Isenegger, John Mason, Jeffrey C. Miller, Joseph F. Petolino, Yidong Ran, Tim Sawbridge, German Spangenberg, Steven R. Webb
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Abstract
Disclosed herein are methods and compositions for parallel or sequential transgene stacking in plants to produce plants with selected phenotypes.
Claims16
A plant cell comprising a targeted genomic modification to one or more alleles of an endogenous acetohydroxyacid synthase (AHAS) gene in the plant cell, wherein:
- (i) the endogenous AHAS gene comprises any one of SEQ ID NO:35-56 or 263-278;
- (ii) the plant cell comprises a site-specific nuclease that binds to a target site as shown in any of SEQ ID NO:35-56 or 263-278 and cleaves the endogenous AHAS gene;
- and
- (iii) the genomic modification comprises an insertion and/or deletion in the endogenous AHAS sequence of the plant cell within any of SEQ ID NO:37-56 or 263-278;
- between SEQ ID NO:35 and 36;
- between SEQ ID NO:37 and 39;
- between SEQ ID NO:38 and 39 or 40;
- between SEQ ID NO:41 and 42;
- between SEQ ID NO:43 and 45;
- between SEQ ID NO:44 and 45;
- between SEQ ID NO:46 and 47;
- between SEQ ID NO:48 and 29;
- between SEQ ID NO:50 and 51;
- between SEQ ID NO:52 or 53 and 54;
- between SEQ ID NO:55 and 56;
- between SEQ ID NO:263 and 264;
- between SEQ ID NO:265 and 266;
- between SEQ ID NO:267 and 268;
- between SEQ ID NO:269 and 270;
- between SEQ ID NO:271 and 272;
- between SEQ ID NO:273 and 274;
- between SEQ ID NO:275 and 276;
- or between SEQ ID NO:277 and 278 of the endogenous AHAS gene following cleavage of the endogenous AHAS gene by the site-specific nuclease.
20 more elements
Dependencies are inferred from the claim text.