Automated instrumentation for production of peptide libraries

An automated method of creating a cell library expressing engineered putative binding peptides on the surface of the cells, the method comprising:

• providing a population of cells;
• processing the population of cells using an instrument for multiplexed nuclease-directed genome editing using introduced nucleic acids and a nuclease to create cells comprising nucleic acids that encode engineered peptides configured to be displayed on a surface of the cells;
• incubating the processed cells to facilitate nucleic acid editing in the cells, wherein the editing provides nucleic acids that encode engineered peptides in the cells;
• and
• allowing the cells to express and display the engineered peptides that are putative target binding peptides on the surface of the cells.

An automated, multiplexed method for identifying peptides that selectively bind one or more targets of interest, the method comprising:

• providing a population of cells;
• processing the population of cells using an automated system for multiplexed nuclease-directed genome editing, wherein the system comprises the steps of:
• introducing nucleic acids that encode engineered peptides and a nuclease to a population of cells;
• incubating the cells to facilitate nucleic acid editing in the cells;
• and
• allowing the edited cells to express and display the engineered peptides on the surface of the edited cells;
• screening the edited cells displaying the engineered peptides against one or more targets of interest;
• and
• identifying the edited cells expressing engineered peptides that selectively bind to one or more targets of interest.