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US 10,501,730 B2Granted
Mannanase variants
AssigneeAB Enzymes Oy
InventorsTaija Leinonen, Leena Valtakari, Michael Seefried, Kari Juntunen, Terhi Puranen
Life of this patent
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Abstract
Variants of mannanase, compositions including the variants, to methods for their production and to methods of using the variants to degrade and modify mannan containing material.
Claims20
A variant of mannanase comprising at least one substitution of an amino acid at a position corresponding to the position 123, 158, 180, 272, 307, or 316 wherein the variant has mannanase activity and is selected from the group consisting of
- 1) a polypeptide having at least 85% sequence identity to residues 27-331 of SEQ ID NO:
- 2;
- 2) a variant encoded by a polynucleotide that hybridizes under high stringency conditions with
- a) nucleotides 79-993 of SEQ ID NO:
- 1 (man 7) or
- b) the full-length complement of a);
- and
- 3) a variant encoded by a polynucleotide having at least 95% sequence identity to the SEQ ID NO:
- 1;
- and wherein the amino acid numbering corresponds to the amino acid numbering of SEQ ID NO:
- 2 full length amino acid sequence containing a signal sequence;
- and wherein the variant comprises at least one additional substitution selected from:
- a substitution which prevents glycosylation of N283;or
- a substitution of 283 or 285;
- or
- a substitution of N283, T285, or S285;
- or
- a substitution of T285, or S285 to a residue other than T or S;
- or
- a substitution of T285, or S285 to the residue A.
17 more elements
The recombinant host cell of 15, wherein the host cell is selected from the group consisting of:
- fungal cells,
- filamentous fungal cells from Division Ascomycota, Subdivision Pezizomycotina;
- from the group consisting of members of the Class Sordariomycetes, Subclass Hypocreomycetidae, Orders Hypocreales and Microascales and Aspergillus, Chrysosporium, Myceliophthora and Humicola;
- Families Hypocreacea, Nectriaceae, Clavicipitaceae, Microascaceae, and Genera Trichoderma (anamorph of Hypocrea), Fusarium, Gibberella, Nectria, Stachybotrys, Claviceps, Metarhizium, Villosiclava, Ophiocordyceps, Cephalosporium, and Scedosporium;
- Trichoderma reesei (Hypocrea jecorina), T. citrinoviridae, T. longibrachiatum, T. virens, T. harzianum, T. asperellum, T. atroviridae, T. parareesei, Fusarium oxysporum, F. gramineanum, F. pseudograminearum, F. venenatum, Gibberella fujikuroi, G. monihformis, G. zeaea, Nectria (Haematonectria) haematococca, Stachybotrys chartarum, S. chlorohalonata, Claviceps purpurea, Metarhizium acridum, M. anisopliae, Villosiclava virens, Ophiocordyceps sinensis, Acremonium (Cephalosporium) chrysogenum, and Scedosporium apiospermum, and Aspergillus niger, Aspergillus awamori, Aspergillus oryzae, Chrysosporium lucknowense, Mycellophthora thermophila, Humicola insolens, and Humicola grisea, bacterial cells, gram positive Bacilli B. subtilis, B. licheniformis, B. megaterium, B. amyloliquefaciens, B. pumilus, gram negative bacteria, Escherichia coli, actinomycetales, Streptomyces sp., and
- yeasts, Saccharomyces cerevisiae, Pichia pastoris, Yarrowia lipolytica,
- Trichoderma reesei or Bacillus.
4 more elements
Dependencies are inferred from the claim text.