Mutant enzymes
AssigneeIsis Innovation Limited
InventorsLuet Lok Wong, Stephen Graham Bell, Christopher Whitehouse
Life of this patent
Estimating the term…
Abstract
This invention relates to mutant enzymes with enhanced properties and processes for oxidation of organic compound substrates using such enzymes.
Claims13
A process for oxidising a substrate which is an organic compound, comprising the step of contacting said organic compound substrate with a mutant CYP102A (Cytochrome P450 family 102A sub-family member) enzyme, wherein said CYP102A enzyme comprises a fusion of a heme monooxygenase domain comprising a P450 fold to a reductase domain, and said mutant CYP102A enzyme comprises substitutions in the polypeptide chain of a wild-type CYP102A enzyme at positions corresponding to amino acid residue positions 307, 47 and 51 of SEQ ID NO:2, thereby enhancing monooxygenase activity and/or altering product selectivity of the mutant enzyme.
Dependencies are inferred from the claim text.