Multivalent and multispecific 41BB-binding fusion proteins
AssigneeInhibrx, Inc.
InventorsBrendan P. Eckelman, John C. Timmer, Chelsie Hata, Kyle S. Jones, Abrahim Hussain, Amir S. Razai, Bryan Becklund, Rajay Pandit, Mike Kaplan, Lucas Rascon, Quinn Deveraux
Life of this patent
Estimating the term…
Abstract
This invention relates generally to molecules that specifically engage 41BB, a member of the TNF receptor superfamily (TNFRSF). More specifically, this invention relates to multivalent and multispecific molecules that bind at least 41BB.
Claims64
An isolated polypeptide comprising at least one VHH domain that binds PDL1 and at least one VHH domain that binds 41BB, wherein each VHH domain that binds PDL1 comprises a CDR1 comprising the amino acid sequence of SEQ ID NO:
- 105, a CDR2 comprising the amino acid sequence of SEQ ID NO:
- 106, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
- 107;
- and wherein each VHH domain that binds 41BB comprises a CDR1, a CDR2, and a CDR3, respectively, comprising the amino acid sequences of SEQ ID NOs:
- 26, 27 and 28;
- or SEQ ID NOs:
- 26, 61, and 28;
- or SEQ ID NOs:
- 26, 63, and 28;
- or SEQ ID NOs:
- 26, 71, and 28;
- or SEQ ID NOs:
- 26, 73, and 28;
- or SEQ ID NOs:
- 26, 75, and 28;
- or SEQ ID NOs:
- 26, 77, and 28;
- or SEQ ID NOs:
- 26, 79, and 28;
- or SEQ ID NOs:
- 65, 61, and 28;
- or SEQ ID NOs:
- 67, 61, and 28;
- or SEQ ID NOs:
- 69, 61, and 28.
An isolated polypeptide comprising a first VHH domain (VHH1) that binds PDL1 and a second VHH domain (VHH2) that binds 41BB, wherein VHH1 comprises a CDR1 comprising the amino acid sequence of SEQ ID NO:
- 105, a CDR2 comprising the amino acid sequence of SEQ ID NO:
- 106, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
- 107;
- and VHH2 comprises a CDR1 comprising the amino acid sequence of SEQ ID NO:
- 26, a CDR2 comprising the amino acid sequence of SEQ ID NO:
- 61, and a CDR3 comprising the amino acid sequence of SEQ ID NO:
- 28;
- and wherein the polypeptide has the structure VHH1-Linker-VHH2-Linker-Hinge-Fc.
Dependencies are inferred from the claim text.