Tau immunotherapy
AssigneeProthena Biosciences Limited
InventorsPeter Seubert, Philip James Dolan, III, Yue Liu, Robin Barbour
Life of this patent
Estimating the term…
Abstract
The invention provides antibodies to tau. The antibodies inhibit or delay tau-associated pathologies and associated symptomatic deterioration.
Claims26
An antibody comprising a mature heavy chain variable region comprising three heavy chain CDRs of the amino acid sequence encoded by SEQ ID NO:25, the mature heavy chain variable region being at least 90% identical to the sequence encoded by SEQ ID NO:25 and a mature light chain variable region comprising three light chain CDRs of SEQ ID NO:22 provided positions L36 and L43 can be occupied by F and S respectively, the mature light chain variable region being at least 90% identical to SEQ ID NO:22.
A method of humanizing an antibody, comprising:
- determining the sequences of the heavy and light chain variable regions of a mouse antibody;
- synthesizing a nucleic acid encoding a humanized heavy chain comprising CDRs of the mouse antibody heavy chain and a nucleic acid encoding a humanized light chain comprising CDRs of the mouse antibody light chain;
- and
- expressing the nucleic acids in a host cell to produce a humanized antibody,
- wherein the mouse antibody is characterized by a mature heavy chain variable region of SEQ ID NO:10 and a mature light chain variable region of SEQ ID NO:16.
A method of producing a humanized, chimeric or veneered antibody, comprising:
- culturing cells transformed with nucleic acids encoding the heavy and light chains of the antibody, so that the cell secretes the antibody;
- and
- purifying the antibody from cell culture media,
- wherein the antibody is a humanized, chimeric or veneered form of a mouse antibody, which is characterized by a mature heavy chain variable region of SEQ ID NO:10 and a mature light chain variable region of SEQ ID NO:16.
A method of producing a cell line producing a humanized, chimeric or veneered antibody, comprising;
- introducing a vector encoding heavy and light chains of an antibody and a selectable marker into cells;
- propagating the cells under conditions to select for cells having increased copy number of the vector;
- isolating single cells from the selected cells;
- and
- banking cells cloned from a single cell selected based on yield of antibody;
- wherein the antibody is a humanized, chimeric or veneered form of a mouse antibody, which is characterized by a mature heavy chain variable region of SEQ ID NO:10 and a mature light chain variable region of SEQ ID NO:16.
Dependencies are inferred from the claim text.