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US 10,501,525 B2Granted
Methods for purification of antibodies using aliphatic alcohols
AssigneeNovartis AG
InventorsAlois Jungbauer, Peter Satzer, Anne-Luise Tscheliessnig
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Abstract
This disclosure relates to methods for isolating antibodies from cell-free culture supernatant.
Claims20
A method for isolating a protein from a cell culture supernatant, the method comprising:
- a) combining a cell culture supernatant with a divalent cation salt under conditions suitable for the precipitation of impurities in the supernatant to produce a primary supernatant;
- b) combining the primary supernatant with an aliphatic alcohol under conditions suitable for forming a protein-containing precipitate and isolating the protein-containing precipitate;
- c) re-suspending the protein-containing precipitate in a buffer comprising a divalent cation salt under conditions suitable for the precipitation of impurities therefrom to produce a protein-containing solution;
- and,
- d) combining the protein-containing solution with an aliphatic alcohol under conditions suitable for forming a purified protein-containing precipitate and isolating the purified protein-containing precipitate.
2 more elements
A method for isolating a protein from a cell culture supernatant, the method comprising:
- a) combining a cell culture supernatant comprising the protein with an aliphatic alcohol under conditions suitable for forming a protein-containing precipitate and isolating the protein-containing precipitate;
- b) re-suspending the protein-containing precipitate in a buffer comprising a divalent cation salt under conditions suitable for the precipitation of impurities therefrom to produce a protein-containing solution;
- and,
- c) combining the protein-containing solution with an aliphatic alcohol under conditions suitable for forming a purified protein-containing precipitate and isolating the purified protein-containing precipitate.
1 more element
Dependencies are inferred from the claim text.