Modified membrane permeability
AssigneesCathay Biotech Inc., Cibt America Inc.
InventorsHoward Chou, Lijun Yu, Xiucai Liu
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Abstract
Provided are microorganisms genetically modified to overexpress porin polypeptides to enhance the production of lysine and lysine derivatives by the microorganism. Also provided are methods of generating such microorganism, and methods of producing lysine and lysine derivatives using the genetically modified microorganisms.
Claims6
A genetically modified host cell comprising a heterologous nucleic acid encoding an OMP porin polypeptide, wherein the host cell overexpresses the OMP porin polypeptide and has increased production of an amino acid lysine and cadaverine, and wherein the OMP porin polypeptide is an OmpA, OmpC, OmpF, OmpX, OmpE, OmpG, or OmpW porin polypeptide set forth as SEQ ID NO:
- 4, 6, 8, 10, 12, 14, or 16 respectively;
- or wherein the OMP porin polypeptide is a conservatively modified porin polypeptide variant of SEQ ID NO:
- 4, 6, 8, 10, 12, 14, or 16 that encodes the mature OMP porin polypeptide.
Dependencies are inferred from the claim text.