Loading patent…
AssigneeThe Regents of the University of California
InventorsErik Rodriguez, John Lin, Roger Tsien, Richard Ting, Geraldine Tran
Estimating the term…
The invention provides evolved red-shifted smURFPs. In some embodiments, the smURFPs are characterized by a) increased Fluorescence of smURFP as compared to infrared FPs IFP1.4 and iRFP713; b) expressing efficiently with minimal toxicity; c) does not require a lyase to covalently attach its chromophore, wherein the chromophore is biliverdin; d) exhibit a wavelengths longer than attainable with jellyfish- or coral-derived FPs using smURFP and IFP2.0; e) allows for functional fusion to hCdt1(30/120) as compared to jellyfish- or coral-derived FPs mAG, eGFP, and mRFP1 which are nonfunctional; and f) allows for deep tissue imaging.
A small ultra-red fluorescent protein (smURFP) variant polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:13, and SEQ ID NO:13 further comprising C52A and A96C mutations.
Dependencies are inferred from the claim text.