Dynamic tissue microfabrication through digital photolithography system and methods

A system for a digitally controlled light based three-dimensional printing of an object comprising:

• a computer operable to generate and control the fabrication of a threedimensional object using digital data of an object and a first specific pattern for particles located within a first layer of the object;
• a projector optically aligned with a build plate;
• a resin vat containing a photopolymerizable resin separated by an electronic gate with a particle reservoir;
• the particle reservoir optically aligned with a holographic optical tweezer, the optical tweezer upon activation capable of producing a plurality of optical traps leading to an accumulation of particles at a focal plane of the optical tweezers in the particle reservoir and capable of moving the optical traps from the particle reservoir to a desired location in the resin vat;
• the projector capable of producing a digital image of desired composition in a layer and selectively photopolymerizing a layer of the resin with a desired pattern.