Method of using methylene blue nanoparticle for detecting cancer by bioimaging

A method of determining a surgical resection margin by using methylene blue nanoparticles for surgically treating of cancer, and the method comprising steps of;

• a2) administrating photosensitive agents containing the methylene blue nanoparticles into a tissue;
• b2) applying light irradiation to the tissue;
• c2) generating a singlet oxygen from the methylene blue nanoparticles;
• and
• d2) analyzing a first image appeared from the tissue and determining a surgical resection margin by comparing the first image with a second image of a known tissue whose condition is normal,
• wherein each of the methylene blue nanoparticles comprises a methylene blue-fatty acid complex and an amphiphilic copolymer of pluronic F-68, wherein the amphiphilic copolymer comprises a polyoxyethylenepolyoxypropylenepolyoxyethylene block copolymer;
• the methylene blue-fatty acid complex is enclosed in a micelle formed by the amphiphilic copolymer;
• and
• each of the methylene blue nanoparticles has a diameter of 80 to 100 nm and is self-assembled in an aqueous environment.