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US 10,500,266 B2Granted
Influenza virus reassortment
AssigneesSeqirus UK Limited, Synthetic Genomics, Inc.
InventorsPeter Mason, Philip Ralph Dormitzer, Heidi Trusheim, Pirada Suphaphiphat
Life of this patent
Estimating the term…
Abstract
New influenza donor strains for the production of reassortant influenza A viruses are provided.
Claims23
A method of preparing a reassortant influenza A virus comprising:
- an HA segment, an NA segment and backbone segments PA, PBI, PB2, NP, NS and M, wherein the backbone segments are from two donor strains, wherein the PB1 segment is from the A/California/07/09 influenza strain and all other backbone segments are from the PR8-X influenza strain, further wherein the PA segment has the sequence of SEQ ID NO:
- 1, the PB2 segment has the sequence of SEQ ID NO:
- 3, the NP segment has the sequence of SEQ ID NO:
- 4, the M segment has the sequence of SEQ ID NO:
- 5, and the NS segment has the sequence of SEQ ID NO:
- 6,
- wherein the method comprises:
- (i) introducing into a culture host at least one expression construct which encode(s) the viral segments required to produce the reassortant influenza A virus;
- and
- (ii) culturing the culture host in order to produce the reassortant influenza A virus.
7 more elements
A method for producing influenza viruses, comprising infecting a culture host with a reassortant influenza A virus comprising an HA segment, an NA segment and backbone segments PA, PB1, PB2, NP, NS and M, wherein the backbone segments are from two donor strains, further wherein at least one backbone segment is from the A/California/07/09 influenza strain and:
- a) the PB2 segment comprises a lysine in the position corresponding to amino acid 389 of SEQ ID NO:
- 3 when aligned to SEQ ID NO:
- 3, using a pairwise alignment algorithm;
- b) the PB2 segment comprises an asparagine in the position corresponding to amino acid 559 of SEQ ID NO:
- 3 when aligned to SEQ ID NO:
- 3, using a pairwise alignment algorithm;
- c) the PA genome segment comprises a lysine in the position corresponding to amino acid 327 of SEQ ID NO:
- 1 when aligned to SEQ ID NO:
- 1, using a pairwise alignment algorithm;
- d) the PA segment comprises an aspartic acid in the position corresponding to amino acid 444 of SEQ ID NO:
- 1 when aligned to SEQ ID NO:
- 1, using a pairwise alignment algorithm;
- e) the PA segment comprises an aspartic acid in the position corresponding to amino acid 675 of SEQ ID NO:
- 1 when aligned to SEQ ID NO:
- 1, using a pairwise alignment algorithm;
- f) the NP segment comprises a threonine in the position corresponding to amino acid 27 of SEQ ID NO:
- 4 when aligned to SEQ ID NO:
- 4 using a pairwise alignment algorithm;
- or
- g) the NP segment comprises an asparagine in the position corresponding to amino acid 375 of SEQ ID NO:
- 4 when aligned to SEQ ID NO:
- 4, using a pairwise alignment algorithm.
19 more elements
Dependencies are inferred from the claim text.