Postpartum cells derived from umbilical cord tissue, and methods of making and using the same
AssigneeDePuy Synthes Products, Inc.
InventorsSanjay Mistry, Anthony J. Kihm, Ian R. Harris, Alexander M. Harmon, Darin J. Messina, Agnieszka Seyda, Chin-Feng Yi, Anna Gosiewska
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Abstract
Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.
Claims19
A cell culture comprising a culture medium and an isolated cell derived from human umbilical cord tissue substantially free of blood, wherein said isolated cell is capable of self-renewal and expansion in culture, has the potential to differentiate into cells of other phenotypes, produces granulocyte chemotactic protein-2 (GCP-2), and can double sufficiently to generate yields of greater than about 1×1017 cells in about 60 days in culture when seeded at about 5,000 cells/cm2 in a flask or yields of greater than about 1×1011 cells in about 60 days in culture when seeded at about 1,000 cells/cm2 in a flask.
A three-dimensional matrix comprising an isolated cell derived from human umbilical cord tissue substantially free of blood, wherein said isolated cell is capable of self-renewal and expansion in culture, has the potential to differentiate into cells of other phenotypes, produces granulocyte chemotactic protein-2 (GCP-2), and can double sufficiently to generate yields of greater than about 1×1017 cells in about 60 days in culture when seeded at about 5,000 cells/cm2 in a flask or yields of greater than about 1×1011 cells in about 60 days in culture when seeded at about 1,000 cells/cm2 in a flask.
A cryopreserved composition comprising an isolated cell derived from human umbilical cord tissue substantially free of blood, wherein said isolated cell is capable of self-renewal and expansion in culture, has the potential to differentiate into cells of other phenotypes, produces granulocyte chemotactic protein-2 (GCP-2), and can double sufficiently to generate yields of greater than about 1×1017 cells in about 60 days in culture when seeded at about 5,000 cells/cm2 in a flask or yields of greater than about 1×1011 cells in about 60 days in culture when seeded at about 1,000 cells/cm2 in a flask.
Dependencies are inferred from the claim text.